{"product_id":"monomethyl-histone-h3-k27-polyclonal-antibody-bs7235","title":"MonoMethyl-Histone H3-K27 Polyclonal Antibody-BS7235","description":"\u003cp\u003eMonoMethyl-Histone H3-K27 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7235-50, BS7235-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P68431\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Other (Wide Range)\u003c\/p\u003e\n\n\u003cp\u003eApplications: DB, WB, IHC, IF\/ICC, ChIP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: DB,1:500 - 1:2000   |   WB,1:500 - 1:2000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200   |   ChIP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Methylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 17KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Enhanced Kit.\u003cbr\u003e\n Exposure time: 180s., Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody., Immunohistochemistry of paraffin-embedded rat kidney using MonoMethyl-Histone H3-K27 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K27 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: H3.4; H3\/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic monomethylated peptide around K27 of human histone H3(NP_003520.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Methylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058780864691,"sku":"BS7235-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058780897459,"sku":"BS7235-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1364b2c8-9473-4592-88f4-24abbfcc7d9f.jpg?v=1708172300","url":"https:\/\/afsbio.com\/products\/monomethyl-histone-h3-k27-polyclonal-antibody-bs7235","provider":"AFSBio Inc.","version":"1.0","type":"link"}