{"product_id":"monomethyl-uhrf1-k385-polyclonal-antibody-bs74109","title":"MonoMethyl-UHRF1-K385 Polyclonal Antibody-BS74109","description":"\u003cp\u003eMonoMethyl-UHRF1-K385 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74109-50, BS74109-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96T88\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: DB, WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: DB,1:500 - 1:2000   |   WB,1:500 - 1:2000   |   IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1\/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Methylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 91kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132 at 37℃ for 90 minutes.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: UHRF1; ICBP90; Np95; RNF106; TDRD22; hNP95; hUHRF1; huNp95\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic monomethylated peptide around K385 of human UHRF1(NP_001041666.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Methylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42975420809395,"sku":"BS74109-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42975420842163,"sku":"BS74109-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6a0b6802-45ee-430b-9b05-7c5dc0be1b12.jpg?v=1705665755","url":"https:\/\/afsbio.com\/products\/monomethyl-uhrf1-k385-polyclonal-antibody-bs74109","provider":"AFSBio Inc.","version":"1.0","type":"link"}