{"product_id":"mouse-4-hne-4-hydroxynonenal-elisa-kit-elk8374","title":"Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit - ELK8374","description":"\u003cp\u003eMouse 4-HNE (4-Hydroxynonenal) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8374-48, ELK8374-96, ELK8374-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.1 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 150 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 2.35-150 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200795500723,"sku":"ELK8374-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200795533491,"sku":"ELK8374-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200795566259,"sku":"ELK8374-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_76c69dfe-2504-4646-8099-ff643371c32f.png?v=1767064060","url":"https:\/\/afsbio.com\/products\/mouse-4-hne-4-hydroxynonenal-elisa-kit-elk8374","provider":"AFSBio Inc.","version":"1.0","type":"link"}