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Mouse aEP (Alpha-Endorphin) ELISA Kit

Mouse aEP (Alpha-Endorphin) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4388

Alternative Names: AEP

Species: Mouse

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 2h

Research: Signal transduction;Endocrinology;Neuro science;Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Endorphin(aEP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Endorphin(aEP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Endorphin(aEP) in the samples is then determined by comparing the OD of the samples to the standard curve.