{"product_id":"mouse-anti-gst-tag-mab-ae001","title":"Mouse Anti GST-Tag mAb - AE001","description":"\u003cp\u003eMouse Anti GST-Tag mAb\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: AE001-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Tag Antibodies\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, ELISA\u003c\/p\u003e\n\n\u003cp\u003eCross-reactivity: Species independent\u003c\/p\u003e\n\n\u003cp\u003eProtein Weight: 26kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved Molecular Weight: 27kDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein (or fragment).\u003c\/p\u003e\n\n\u003cp\u003eSWISS: P08515\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: GST;GST tag;GST-tag\u003c\/p\u003e\n\n\u003cp\u003eSource: Mouse\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG1\u003c\/p\u003e\n\n\u003cp\u003ePurity: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Avoid freeze \/ thaw cycles. Buffer: PBS containing 50% glycerol and 1.5% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eRecommended Dilutions: WB, 1:2000 - 1:10000 IP, 3μg antibody for 1μg extracts of recombinant protein ELISA, Recommended starting concentration is 1 μg\/mL. Please optimize the concentration based on your specific assay requirements.\u003c\/p\u003e\n\n\u003cp\u003eNCBI Alias: GST\u003c\/p\u003e\n\n\u003cp\u003eClone Number Info: AMC0501\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"Default Title","offer_id":45190101008563,"sku":"AE001-100","price":231.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_472f77fb-850c-4e94-9e19-0db9fd6e2409.png?v=1766442473","url":"https:\/\/afsbio.com\/products\/mouse-anti-gst-tag-mab-ae001","provider":"AFSBio Inc.","version":"1.0","type":"link"}