{"product_id":"mouse-anti-klh-igm-keyhole-limpet-hemocyanin-igm-elisa-kit-elk0110","title":"Mouse Anti-KLH IgM (Keyhole Limpet Hemocyanin IgM) ELISA Kit - ELK0110","description":"\u003cp\u003eMouse Anti-KLH IgM (Keyhole Limpet Hemocyanin IgM) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0110-48, ELK0110-96, ELK0110-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Keyhole limpet IgM\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.51 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Immunology\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgM in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43682366881971,"sku":"ELK0110-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":44555572412595,"sku":"ELK0110-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200629268659,"sku":"ELK0110-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_60e652c5-a7d6-4dff-889a-bd2dbb786ac2.png?v=1767060212","url":"https:\/\/afsbio.com\/products\/mouse-anti-klh-igm-keyhole-limpet-hemocyanin-igm-elisa-kit-elk0110","provider":"AFSBio Inc.","version":"1.0","type":"link"}