Mouse Anti-NS3 (JFH-1) Monoclonal Clone 100μg (Monoclonal)

Mouse Anti-NS3 (JFH-1) Monoclonal Clone 100μg (Monoclonal)

Regular price
$775.92
Regular price
Sale price
$775.92
Unit price
per 
Availability
Sold out

Clone: 2E3

Volume: 100μl (1 μg/μl)

Specificity: Recognizes the helicase domain of JFH-1 NS3 (genotype 2a)

Cross-reactivity: Cross-reactive against genotype 1a

Form: Protein A-purified

Origin: Mouse

Isotype: IgG1

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

Storage: 

  • Short Term: 4°C
  • Long Term (up to 1 year): -20°C (avoid repeated freeze/thaw)

Applications: Western, IF, IP, FACS, and TCID50 assay

Working Dilutions: 

  • TCID50:   1:1000-1:5000
  • Western Blot:   1:1000-1:5000
  • IF:   1:1000
  • FACS:   1:500

Figures



Fig. 1. Cell lysate run on 12% SDS-PAGE and transferred to PVDF.
Primary antibody incubation performed for 1-hour at room temperature.
(overnight incubations also suitable at 4°C).


Fig. 2

 

References:

  1. Fahmy et al. Sci Rep. 2017 Jan 9;7:40351. doi: 10.1038/srep40351.
  2. Chong et al. J Biol Chem. 2016 Feb 19;291(8):3918-31. doi: 10.1074/jbc.M115.675413. Epub 2015 Dec 23.
  3. Perez-Berna et al. ACS Nano. 2016 Jul 26;10(7):6597-611.
  4. Saeed et al. Nature. 2015 Aug 27;524(7566):471-5. doi: 10.1038/nature14899. Epub 2015 Aug 12.
  5. Afzal et al. J Gen Virol. 2015 Feb;96(Pt 2):311-21. doi: 10.1099/vir.0.070433-0. Epub 2014 Oct 28.
  6. Brennd rfer et al. J Immunol. 2014 Feb 15;192(4):1671-80. doi: 10.4049/jimmunol.1301077. Epub 2014 Jan 17.
  7. Paul et al. J Virol. 2013 Oct;87(19):10612-27. doi: 10.1128/JVI.01370-13. Epub 2013 Jul 24.
  8. Friesland et al. J Virol. 2013 Jun;87(11):6377-90. doi: 10.1128/JVI.03557-12. Epub 2013 Mar 27.
  9. Fridell et al. J Virol. 2013 Feb;87(4):2320-9.
  10. Wu et al. PLoS Pathog. 2012;8(4):e1002617. doi: 10.1371/journal.ppat.1002617. Epub 2012 Apr 5.
  11. Jirasko et al. PLoS Pathog. 2010 Dec 16;6(12):e1001233.
  12.  Backes et al. J Virol. 2010 Jun;84(11):5775-89
  13. Kenworthy et al. Nucleic Acids Res. 2009 Oct;37(19):6587-99.
  14. Kaul et al. PLoS Pathog. 2009 Aug;5(8):e1000546
  15. Yang et al. J Virol. 2008 Jun;82(11):5269-78.