Catalogue Number: ELK4629
Alternative Names: GPR2; G Protein-Coupled Receptor 2
Species: Mouse
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 10(CCR10). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 10(CCR10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 10(CCR10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 10(CCR10) in the samples is then determined by comparing the OD of the samples to the standard curve.