{"product_id":"mouse-col3a1-collagen-type-iii-alpha-1-microsample-elisa-kit-elk10813ms","title":"Mouse COL3a1 (Collagen Type III Alpha 1) Microsample ELISA Kit - ELK10813MS","description":"\u003cp\u003eMouse COL3a1 (Collagen Type III Alpha 1) Microsample ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10813MS-48, ELK10813MS-96, ELK10813MS-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Mouse\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 16.7 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 4000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 62.5-4000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cytokine; Tumor immunity; Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45199970271411,"sku":"ELK10813MS-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45199970304179,"sku":"ELK10813MS-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45199970336947,"sku":"ELK10813MS-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_6b0ffc84-17b5-4f12-9ec4-cb8b05e1766e.png?v=1767057253","url":"https:\/\/afsbio.com\/products\/mouse-col3a1-collagen-type-iii-alpha-1-microsample-elisa-kit-elk10813ms","provider":"AFSBio Inc.","version":"1.0","type":"link"}