{"product_id":"mouse-f1-2-prothrombin-fragment-1-2-elisa-kit-elk2869","title":"Mouse F1+2 (Prothrombin Fragment 1+2) ELISA Kit - ELK2869","description":"\u003cp\u003eMouse F1+2 (Prothrombin Fragment 1+2) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2869-48, ELK2869-96, ELK2869-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: F1+2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.111 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Hematology\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211654168755,"sku":"ELK2869-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211654201523,"sku":"ELK2869-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200721543347,"sku":"ELK2869-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_6a42b76f-140d-4054-9e29-d36214ee75bd.png?v=1767062487","url":"https:\/\/afsbio.com\/products\/mouse-f1-2-prothrombin-fragment-1-2-elisa-kit-elk2869","provider":"AFSBio Inc.","version":"1.0","type":"link"}