{"product_id":"mouse-hif-1α-hypoxia-inducible-factor-1-alpha-clia-kit-e-cl-m0424","title":"Mouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit - E-CL-M0424","description":"\u003cp\u003eMouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-CL-M0424-24, E-CL-M0424-48, E-CL-M0424-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: HIF-1α\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q61221\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonyms: HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor;\u003c\/p\u003e \n\n\u003cp\u003eResearch Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplication: CLIA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of Mouse HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Tissue homogenates,cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eType: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Chemiluminescence\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 18.75 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 31.25-2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HIF-1α. You can calculate the concentration of Mouse HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Mouse HIF-1α in samples. No significant cross-reactivity or interference between Mouse HIF-1α and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 6 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43038021681331,"sku":"E-CL-M0424-24","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43038021714099,"sku":"E-CL-M0424-48","price":764.4,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43038021746867,"sku":"E-CL-M0424-96","price":954.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_df481498-d117-440a-992c-7762cf89e02e.jpg?v=1707481382","url":"https:\/\/afsbio.com\/products\/mouse-hif-1%ce%b1-hypoxia-inducible-factor-1-alpha-clia-kit-e-cl-m0424","provider":"AFSBio Inc.","version":"1.0","type":"link"}