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Mouse MGP (Matrix Gla Protein) ELISA Kit

Mouse MGP (Matrix Gla Protein) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4554

Alternative Names: MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

Species: Mouse

Assay Type: Sandwich

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Metabolic pathway;Hematology;Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Gla Protein(MGP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Gla Protein(MGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Gla Protein(MGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Gla Protein(MGP) in the samples is then determined by comparing the OD of the samples to the standard curve.