Catalogue Number: ELK4556
Alternative Names: CD10; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase
Assay Type: Sandwich
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin(NEP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neprilysin(NEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neprilysin(NEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neprilysin(NEP) in the samples is then determined by comparing the OD of the samples to the standard curve.