{"product_id":"mouse-nfκb-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk8449","title":"Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit - ELK8449","description":"\u003cp\u003eMouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8449-48, ELK8449-96, ELK8449-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 11.5 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 31.25-2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Apoptosis;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q04207\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213918437555,"sku":"ELK8449-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213918470323,"sku":"ELK8449-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201411408051,"sku":"ELK8449-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_6fc2dce9-bd73-4e90-9c19-0a566a38fae8.png?v=1767096454","url":"https:\/\/afsbio.com\/products\/mouse-nf%ce%bab-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk8449","provider":"AFSBio Inc.","version":"1.0","type":"link"}