{"product_id":"mouse-nfκb-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk9154","title":"Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit - ELK9154","description":"\u003cp\u003eMouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9154-48, ELK9154-96, ELK9154-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NF kappa B p65delta3; NFKB3\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 12.2 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 31.25-2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Immunology\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q04207\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213959987379,"sku":"ELK9154-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213960020147,"sku":"ELK9154-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201421041843,"sku":"ELK9154-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_47ae6126-40e4-440c-81f3-bbc1dc3c93fa.png?v=1767096908","url":"https:\/\/afsbio.com\/products\/mouse-nf%ce%bab-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk9154","provider":"AFSBio Inc.","version":"1.0","type":"link"}