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Mouse OX (Orexin) ELISA Kit

Mouse OX (Orexin) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4723

Alternative Names: HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor

Species: Mouse

Assay Type: Competitive Inhibition

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research: Neuro science;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Orexin(OX) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Orexin(OX). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Orexin(OX) in the samples is then determined by comparing the OD of the samples to the standard curve.