{"product_id":"mouse-phospho-insulin-receptor-β-subunit-ptyr1162-1163-elisa-kit-elk0766","title":"Mouse phospho-Insulin Receptor, β Subunit (pTyr1162\/1163) ELISA Kit - ELK0766","description":"\u003cp\u003eMouse phospho-Insulin Receptor, β Subunit (pTyr1162\/1163) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0766-48, ELK0766-96, ELK0766-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: p-IR\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.68 U\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 U\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.57-100 U\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Endocrinology; Autoimmunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162\/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162\/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162\/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162\/1163] in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211531681971,"sku":"ELK0766-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211531714739,"sku":"ELK0766-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200642932915,"sku":"ELK0766-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_2ff82016-fea5-4b2a-876c-3222c092fe3f.png?v=1767060749","url":"https:\/\/afsbio.com\/products\/mouse-phospho-insulin-receptor-%ce%b2-subunit-ptyr1162-1163-elisa-kit-elk0766","provider":"AFSBio Inc.","version":"1.0","type":"link"}