{"product_id":"mouse-piezo-1-piezo-type-mmechanosensitive-iion-channel-component-1-elisa-kit-elk0282","title":"Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit - ELK0282","description":"\u003cp\u003eMouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0282-48, ELK0282-96, ELK0282-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: PIEZO1; FAM38A; KIAA0233\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 22.3 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: E2JF22\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212878905523,"sku":"ELK0282-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212878938291,"sku":"ELK0282-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200859529395,"sku":"ELK0282-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_381ba0de-4742-4f6f-a140-ace83ed9fbc0.png?v=1767065700","url":"https:\/\/afsbio.com\/products\/mouse-piezo-1-piezo-type-mmechanosensitive-iion-channel-component-1-elisa-kit-elk0282","provider":"AFSBio Inc.","version":"1.0","type":"link"}