{"product_id":"mouse-pnf-h-phosphorylated-neurofilament-h-elisa-kit-elk7624","title":"Mouse pNF-H (Phosphorylated Neurofilament H) ELISA Kit - ELK7624","description":"\u003cp\u003eMouse pNF-H (Phosphorylated Neurofilament H) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK7624-48, ELK7624-96, ELK7624-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NFH; 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; Neurofilament, Heavy Polypeptide\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 5.6 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 15.63-1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212128977075,"sku":"ELK7624-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212129009843,"sku":"ELK7624-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200762077363,"sku":"ELK7624-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_961639dd-6c11-44c2-8388-488b961097a5.png?v=1767063574","url":"https:\/\/afsbio.com\/products\/mouse-pnf-h-phosphorylated-neurofilament-h-elisa-kit-elk7624","provider":"AFSBio Inc.","version":"1.0","type":"link"}