{"product_id":"mouse-rasgap-ras-gtpase-activating-protein-elisa-kit-e-el-m1005","title":"Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit - E-EL-M1005","description":"\u003cp\u003eMouse RasGAP (Ras GTPase Activating Protein) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-EL-M1005-24, E-EL-M1005-48, E-EL-M1005-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: RasGAP\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: F6SEU4\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonyms: Ras GTPase-activating protein 1; RASA1; GAP; GTPase-activating protein; RasGAP; Ras p21 protein activator; p120GAP; GAP; RASA;\u003c\/p\u003e \n\n\u003cp\u003eResearch Areas: Cancer, Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of Mouse RasGAP concentrations in Serum, plasma and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, Plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eType: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 46.88 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RasGAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RasGAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RasGAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RasGAP. You can calculate the concentration of Mouse RasGAP in the samples by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Mouse RasGAP in samples. No significant cross-reactivity or interference between Mouse RasGAP and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 12 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43012373348531,"sku":"E-EL-M1005-24","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43012373381299,"sku":"E-EL-M1005-48","price":681.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43012373414067,"sku":"E-EL-M1005-96","price":852.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_4620278f-250e-426d-af94-5717c7f8f7aa.jpg?v=1706704255","url":"https:\/\/afsbio.com\/products\/mouse-rasgap-ras-gtpase-activating-protein-elisa-kit-e-el-m1005","provider":"AFSBio Inc.","version":"1.0","type":"link"}