{"product_id":"mouse-smad2-3-mothers-against-decapentaplegic-homolog-2-3-elisa-kit-elk9883","title":"Mouse Smad2\/3 (Mothers Against Decapentaplegic Homolog 2\/3) ELISA Kit - ELK9883","description":"\u003cp\u003eMouse Smad2\/3 (Mothers Against Decapentaplegic Homolog 2\/3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9883-48, ELK9883-96, ELK9883-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Smad 2\/3\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.059 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Apoptosis; Tumor immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2\/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2\/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2\/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Smad2\/3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212866584755,"sku":"ELK9883-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212866617523,"sku":"ELK9883-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200836198579,"sku":"ELK9883-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_54533652-2c33-4b9f-b49f-4c880c08137c.png?v=1767065322","url":"https:\/\/afsbio.com\/products\/mouse-smad2-3-mothers-against-decapentaplegic-homolog-2-3-elisa-kit-elk9883","provider":"AFSBio Inc.","version":"1.0","type":"link"}