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Mouse VTN (Vitronectin) ELISA Kit

Mouse VTN (Vitronectin) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4697

Alternative Names: V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

Species: Mouse

Assay Type: Sandwich

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Plasma.

Assay Length: 3.5h

Research: Signal transduction;Metabolic pathway;Tumor immunity;Hepatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitronectin(VTN). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitronectin(VTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitronectin(VTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitronectin(VTN) in the samples is then determined by comparing the OD of the samples to the standard curve.