{"product_id":"mouse-vwf-von-willebrand-factor-elisa-kit-elk10104","title":"Mouse vWF (Von Willebrand Factor) ELISA Kit - ELK10104","description":"\u003cp\u003eMouse vWF (Von Willebrand Factor) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10104-48, ELK10104-96, ELK10104-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: F8VWF; VWD; von Willebrand antigen 2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.049 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: plasma\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Hematology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse vWF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q8CIZ8\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200878272691,"sku":"ELK10104-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200878305459,"sku":"ELK10104-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200878338227,"sku":"ELK10104-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_aeba4949-0707-4cb0-85bd-18c4fd21233a.png?v=1767066349","url":"https:\/\/afsbio.com\/products\/mouse-vwf-von-willebrand-factor-elisa-kit-elk10104","provider":"AFSBio Inc.","version":"1.0","type":"link"}