{"product_id":"mrps7-polyclonal-antibody-bs77120","title":"MRPS7 Polyclonal Antibody-BS77120","description":"MRPS7 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS77120-50, BS77120-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q9Y2R9\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\n\nBackground: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 28kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using MRPS7 Rabbit pAb at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 90s., Immunofluorescence analysis of C6 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\n\nAlternative Name: MRPS7; MRP-S; MRP-S7; RP-S7; RPMS7; S7mt; bMRP27a\n\nImmunogen: Recombinant fusion protein of human MRPS7(NP_057055.2).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047643971763,"sku":"BS77120-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047644004531,"sku":"BS77120-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_31d16bd7-ef21-4561-bf85-9f4e82859283.jpg?v=1707826672","url":"https:\/\/afsbio.com\/products\/mrps7-polyclonal-antibody-bs77120","provider":"AFSBio Inc.","version":"1.0","type":"link"}