{"product_id":"msh6-monoclonal-antibody-mb0153","title":"MSH6 Monoclonal Antibody-MB0153","description":"\u003cp\u003eMSH6 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0153-50, MB0153-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P52701\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:300~500\u003cbr\u003e\nICC: 1:50~100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Component of the post-replicative DNA mismatch repair system (MMR).Heterodimerizes with MSH2 to form MutS alpha,which binds to DNA mismatches thereby initiating DNA repair.When bound,MutS alpha bends the DNA helix and shields approximately 20 base pairs,and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.After mismatch binding,forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events,including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP--\u0026gt;ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of MSH6 and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 160KDa\u003cbr\u003e\nObserved band size: 160KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of MSH6 antibody in 293 and HCT116 cell lysates using MSH6 antibody (1:300-1:500 diluted)., Immunocytochemistry staining of HeLa cells using anti-MSH6 antibody (dilution 1:100).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA mismatch repair protein Msh6; hMSH6; G\/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160; MSH6; GTBP\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human MSH6.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989567082675,"sku":"MB0153-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989567115443,"sku":"MB0153-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b314e2a3-ee74-4caf-9367-8acb9b0ac909.jpg?v=1706095490","url":"https:\/\/afsbio.com\/products\/msh6-monoclonal-antibody-mb0153","provider":"AFSBio Inc.","version":"1.0","type":"link"}