{"product_id":"multiplex-assay-kit-for-glutamine-fructose-6-phosphate-transaminase-1-gfpt1-etc-by-flia-flow-luminescence-immunoassay-lmd865hu","title":"Multiplex Assay Kit for Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay) - LMD865Hu","description":"Multiplex Assay Kit for Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay)\n\nSize: 96T\n\nCatalogue Number: LMD865Hu-96\n\nCitations, Manuals and MSDS Available upon request.\n\nDetection range: 0.0098-10ng\/mL\n\nApplication: FLIA Kit for Antigen Detection.\n\nOrganism species: Homo sapiens (Human)\n\nSensitivity: The minimum detectable dose of this kit is typically less than 0.0033ng\/mL\n\nSample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids\n\nAssay length: 3h\n\nMethod: Double-antibody Sandwich\n\nSpecificity: This assay has high sensitivity and excellent specificity for detection of Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.\n\nPrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamine Fructose-6-Phosphate Transaminase 1 (GFPT1), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD\/meanX100 Intra-Assay: CV\u0026lt;10% Inter-Assay: CV\u0026lt;12% \n\nAssay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;\n2. Add 100μL standard or sample to each well, \n add 10μL magnetic beads, and incubate 90min at 37°C on shaker;\n3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;\n4. Wash plate on magnetic frame for three times;\n5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;\n6. Wash plate on magnetic frame for three times;\n7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.\n\nTest principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.\n\nAlternative Names: GFAT; GFA; •D-fructose-6-phosphate amidotransferase 1; Hexosephosphate aminotransferase 1; Glutamine-fructose-6-phosphate aminotransferase isomerizing 1 \n\nItem Name: Glutamine Fructose-6-Phosphate Transaminase 1\n\nResearch Field: Enzyme \u0026amp; Kinase; \n\nUniprot: Q06210\n\nResearch Use Only","brand":"Cloud-Clone","offers":[{"title":"Default Title","offer_id":45372087795891,"sku":"LMD865Hu-96","price":1149.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/multiplex-assay-kit-for-glutamine-fructose-6-phosphate-transaminase-1-gfpt1-etc-by-flia-flow-luminescence-immunoassay-lmd865hu","provider":"AFSBio Inc.","version":"1.0","type":"link"}