{"product_id":"multiplex-assay-kit-for-growth-arrest-and-dna-damage-inducible-protein-gamma-gadd45g-etc-by-flia-flow-luminescence-immunoassay-lml536mu","title":"Multiplex Assay Kit for Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay) - LML536Mu","description":"\u003cp\u003eMultiplex Assay Kit for Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay)\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: LML536Mu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDetection range: 0.04-40ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eApplication: FLIA Kit for Antigen Detection.\u003c\/p\u003e\n\n\u003cp\u003eOrganism species: Mus musculus (Mouse)\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: The minimum detectable dose of this kit is typically less than 0.013 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample type: Tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay length: 3h\u003c\/p\u003e\n\n\u003cp\u003eMethod: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD\/meanX100 Intra-Assay: CV\u0026lt;10% Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e \n\n\u003cp\u003eAssay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;\u003cbr\u003e\n2. Add 100μL standard or sample to each well, \u003cbr\u003e\n add 10μL magnetic beads, and incubate 90min at 37°C on shaker;\u003cbr\u003e\n3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;\u003cbr\u003e\n4. Wash plate on magnetic frame for three times;\u003cbr\u003e\n5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;\u003cbr\u003e\n6. Wash plate on magnetic frame for three times;\u003cbr\u003e\n7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CR6; DDIT2; GADD45gamma; GRP17; Gadd-Related Protein, 17 kD; Cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein\u003c\/p\u003e\n\n\u003cp\u003eItem Name: Growth Arrest And DNA Damage Inducible Protein Gamma\u003c\/p\u003e\n\n\u003cp\u003eResearch Field: Signal transduction;\u003c\/p\u003e \n\n\u003cp\u003eUniprot: Q9Z111\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"Default Title","offer_id":45379447980211,"sku":"LML536Mu-96","price":1330.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/multiplex-assay-kit-for-growth-arrest-and-dna-damage-inducible-protein-gamma-gadd45g-etc-by-flia-flow-luminescence-immunoassay-lml536mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}