{"product_id":"multiplex-assay-kit-for-protein-disulfide-isomerase-pdi-etc-by-flia-flow-luminescence-immunoassay-lmb498mu","title":"Multiplex Assay Kit for Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay) - LMB498Mu","description":"\u003cp\u003eMultiplex Assay Kit for Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay)\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: LMB498Mu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDetection range: 0.01-10ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eApplication: FLIA Kit for Antigen Detection.\u003c\/p\u003e\n\n\u003cp\u003eOrganism species: Mus musculus (Mouse)\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: The minimum detectable dose of this kit is typically less than 0.003 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample type: Tissue homogenates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay length: 3h\u003c\/p\u003e\n\n\u003cp\u003eMethod: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD\/meanX100 Intra-Assay: CV\u0026lt;10% Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e \n\n\u003cp\u003eAssay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;\u003cbr\u003e\n2. Add 100μL standard or sample to each well, \u003cbr\u003e\n add 10μL magnetic beads, and incubate 90min at 37°C on shaker;\u003cbr\u003e\n3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;\u003cbr\u003e\n4. Wash plate on magnetic frame for three times;\u003cbr\u003e\n5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;\u003cbr\u003e\n6. Wash plate on magnetic frame for three times;\u003cbr\u003e\n7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: PDI-A2; PDA2, PDIA2, PDIR; PDIP; Protein Disulfide Isomerase, Pancreatic; Protein Disulfide Isomerase-Associated 2; Pancreas-specific protein disulfide isomerase\u003c\/p\u003e\n\n\u003cp\u003eItem Name: Protein Disulfide Isomerase\u003c\/p\u003e\n\n\u003cp\u003eResearch Field: Enzyme \u0026amp; Kinase; Metabolic pathway; Hepatology;\u003c\/p\u003e \n\n\u003cp\u003eUniprot: D3Z6P0\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"Default Title","offer_id":45379469115571,"sku":"LMB498Mu-96","price":1149.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/multiplex-assay-kit-for-protein-disulfide-isomerase-pdi-etc-by-flia-flow-luminescence-immunoassay-lmb498mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}