{"product_id":"multiplex-assay-kit-for-proteoglycan-4-prg4-etc-by-flia-flow-luminescence-immunoassay-lmg246mu","title":"Multiplex Assay Kit for Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay) - LMG246Mu","description":"\u003cp\u003eMultiplex Assay Kit for Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay)\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: LMG246Mu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eDetection range: 0.01-10ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eApplication: FLIA Kit for Antigen Detection.\u003c\/p\u003e\n\n\u003cp\u003eOrganism species: Mus musculus (Mouse)\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: The minimum detectable dose of this kit is typically less than 0.003 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample type: Serum, plasma, synovial fluid and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay length: 3h\u003c\/p\u003e\n\n\u003cp\u003eMethod: Double-antibody Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteoglycan 4 (PRG4), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD\/meanX100 Intra-Assay: CV\u0026lt;10% Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e \n\n\u003cp\u003eAssay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;\u003cbr\u003e\n2. Add 100μL standard or sample to each well, \u003cbr\u003e\n add 10μL magnetic beads, and incubate 90min at 37°C on shaker;\u003cbr\u003e\n3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;\u003cbr\u003e\n4. Wash plate on magnetic frame for three times;\u003cbr\u003e\n5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;\u003cbr\u003e\n6. Wash plate on magnetic frame for three times;\u003cbr\u003e\n7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: MSF; CACP; HAPO; JCAP; SZP; Megakaryocyte Stimulating factor; Articular Superficial Zone Protein; Camptodactyly; Arthropathy, Coxa Vara, Pericarditis Syndrome\u003c\/p\u003e\n\n\u003cp\u003eItem Name: Proteoglycan 4\u003c\/p\u003e\n\n\u003cp\u003eResearch Field: Metabolic pathway;\u003c\/p\u003e \n\n\u003cp\u003eUniprot: Q9JM99\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"Default Title","offer_id":45379473473715,"sku":"LMG246Mu-96","price":1209.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/multiplex-assay-kit-for-proteoglycan-4-prg4-etc-by-flia-flow-luminescence-immunoassay-lmg246mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}