{"product_id":"myf-6-r150-polyclonal-antibody-bs1956","title":"Myf-6 (R150) Polyclonal Antibody-BS1956","description":"\u003cp\u003eMyf-6 (R150) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1956-50, BS1956-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P23409\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eBackground: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Myf-6 (R150) polyclonal antibody detects endogenous levels of Myf-6 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Myf-6 (R150) pAb at 1:1000 dilution\u003cbr\u003e\nLane 1: THP-1 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: MG63 whole cell lysate (20ug)\u003cbr\u003e\nLane 3: PC3 whole cell lysate (10ug)\u003cbr\u003e\nLane 4: H9C2 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: SP2\/0 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of Myf-6 (R150) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 30 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Myogenic factor 6; Myf-6; Class C basic helix-loop-helix protein 4; bHLHc4; Muscle-specific regulatory factor 4; MYF6; BHLHC4; MRF4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human Myf-6.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953217769651,"sku":"BS1956-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953217802419,"sku":"BS1956-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7f9944e7-d754-443d-adac-db3cb6134ee7.jpg?v=1705056970","url":"https:\/\/afsbio.com\/products\/myf-6-r150-polyclonal-antibody-bs1956","provider":"AFSBio Inc.","version":"1.0","type":"link"}