{"product_id":"nadp-nadph-colorimetric-assay-kit-wst-8-e-bc-k803-m","title":"NADP+\/NADPH Colorimetric Assay Kit (WST-8) - E-BC-K803-M","description":"\u003cp\u003eNADP+\/NADPH Colorimetric Assay Kit (WST-8)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-K803-M-96\u003c\/p\u003e\n\n\u003cp\u003eLead times: 2-3 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Detect total content of NADP+and NADPH:\u003cbr\u003e\nGlucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+and NADPH can be quantified by measure the OD value at 450 nm.\u003cbr\u003e\nDetect NADPH:\u003cbr\u003e\nAfter treating sample, heat at 60℃ water bath for 30 min. the NADP+of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm.\u003cbr\u003e\nDetect NADP+and NADP+\/NADPH:\u003cbr\u003e\nThe content of NADP+and the ratio of NADP+\/NADPH in the sample can be obtained according to the total content of NADP+and NADPH obtained of the first two steps as well as the separate content of NADPH.\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: NADP;NADPH\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Oxidative Stress;Metabolic Diseases;Glycolysis and Lipid Metabolism;Cuproptosis;Disulfidptosis\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.02-5.0 μmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Animal tissue;Cell\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 1 h 10 min\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.02 μmol\/L\u003c\/p\u003e\n\n\u003cp\u003eInter cv: 5.5%\u003c\/p\u003e\n\n\u003cp\u003eIntra cv: 2.1%\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"96T","offer_id":46264398446771,"sku":"E-BC-K803-M-96","price":701.25,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Screenshot_2024-02-12_153513_49a86b0b-f422-42b0-baac-7435bb35d3d6.png?v=1707826183","url":"https:\/\/afsbio.com\/products\/nadp-nadph-colorimetric-assay-kit-wst-8-e-bc-k803-m","provider":"AFSBio Inc.","version":"1.0","type":"link"}