NativeFolder Bacterial Culture Medium Kit - 1 kit (1L)
NativeFolder Bacterial Culture Medium Kit - 1 kit (1L)
NativeFolder Bacterial Culture Medium Kit - 1 kit (1L)

NativeFolder Bacterial Culture Medium Kit - 1 kit (1L)

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NativeFolderTM is a bacterial culture medium specially formulated to promote the folding and the expression of soluble recombinant  proteins in E. coli. NativeFolderTM contains proprietary compounds that prevent protein aggregation and misfolding during protein expression. Don’t waste your time experimenting with the renaturation of inclusion bodies. Use NativeFolderTM and express your correctly folded target protein in the soluble fraction. Simply add water to the provided mix, autoclave and filter sterilize. Recombinant proteins of up to 110 kDa were successfully expressed as soluble fractions using NativeFolderTM.

 

Product Data Sheet Download PDF 
MSDS Download PDF
Catalog # K1010004
Content NativeFolderTM Mix A

 

NativeFolderTM Mix B

Instructions

Keywords Culture Medium, folding, solubilization, recombinant proteins
Storage Room temperature
Expression Protocol
  1. Dissolve NativeFolderTM Mix A in 0.75 L of di-ionized water, stir until completely dissolved and autoclave.
  2. Dissolve NativeFolderTM Mix B in 0.25 L of di-ionized water, stir until completely dissolved filter sterilize before use (DO NOT AUTOCLAVE).
  3.  Combine 0.75 L of NativeFolderTM Mix A with 0.25 L of NativeFolder Mix B to make 1.0 L of NativeFolderTM Culture Medium. Follow Steps 4-10.