{"product_id":"ncoa-3-rabbit-polyclonal-antibody-es4237","title":"NCoA-3 Rabbit Polyclonal Antibody - ES4237","description":"\u003cp\u003eNCoA-3 Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES4237-50, ES4237-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300\/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: NCOA3, AIB1, BHLHE42, RAC3, TRAM1, Nuclear receptor coactivator 3, NCoA-3, ACTR, Amplified in breast cancer 1 protein, AIB-1, CBP-interacting protein, pCIP, Class E basic helix-loop-helix protein 42, bHLHe42, Receptor-associated coactivator 3, RAC-3, Steroid receptor coactivator protein 3, SRC-3, Thyroid hormone receptor activator molecule 1, TRAM-1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized peptide derived from the Internal region of human NCoA-3.\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: NCoA-3\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: NCoA-3 Polyclonal Antibody detects endogenous levels of NCoA-3 protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. IHC-p: 1\/100-1\/300. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed, High expression in heart, skeletal muscle, pancreas and placenta, Low expression in brain, and very low in lung, liver and kidney,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 160kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Endocrine resistance, Estrogen signaling pathway, Thyroid hormone signaling pathway, Pathways in cancer, Breast cancer\u003c\/p\u003e\n\n\u003cp\u003eFunction: Alternative Products: Additional isoforms seem to exist,Catalytic Activity: Acetyl-CoA + histone = CoA + acetylhistone\u003cbr\u003e\nDomain: Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain)\u003cbr\u003e\nEnzyme Regulation: Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors\u003cbr\u003e\nFunction: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubuni\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm Nucleus Mainly cytoplasmic and weakly nuclear Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: NCOA3\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Nuclear receptor coactivator 3\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: O09000\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q9EPU2\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46316566675635,"sku":"ES4237-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46316566708403,"sku":"ES4237-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/ncoa-3-rabbit-polyclonal-antibody-es4237","provider":"AFSBio Inc.","version":"1.0","type":"link"}