{"product_id":"nelfe-monoclonal-antibody-mb11292","title":"NELFE Monoclonal Antibody-MB11292","description":"\u003cp\u003eNELFE Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB11292-50, MB11292-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P18615\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IP: 1\/20\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 43 kDa; Observed MW: 43 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of NELFe in C6 lysates using NELFE antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NELFe antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RD; RDP; RDBP; D6S45; NELF-E\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human NELFe\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42975242387635,"sku":"MB11292-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42975242420403,"sku":"MB11292-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d389b535-ea37-46a4-8c5b-ec209c2f8765.jpg?v=1705662181","url":"https:\/\/afsbio.com\/products\/nelfe-monoclonal-antibody-mb11292","provider":"AFSBio Inc.","version":"1.0","type":"link"}