{"product_id":"nephrin-h892-polyclonal-antibody-bs3462","title":"Nephrin (H892) Polyclonal Antibody-BS3462","description":"\u003cp\u003eNephrin (H892) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3462-50, BS3462-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O60500\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eBackground: Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.\u003c\/p\u003e \n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Nephrin (H892) polyclonal antibody detects endogenous levels of Nephrin protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Nephrin (H892) polyclonal antibody at 1:1000 dilution   Lane 1: The muscle tissue lysate of Rat (47ug)   Lane 2: HEK293T whole cell lysate (41ug)  Lane 3: Jurkat whole cell lysate (41ug)       \u003cbr\u003e\nLane 4: H9C2 whole cell lysate (60ug) , Immunohistochemistry (IHC) analyzes of Nephrin (H892) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 135 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953235792051,"sku":"BS3462-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953235824819,"sku":"BS3462-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f5e82eaa-c069-41fc-b523-f23034a7b179.jpg?v=1705057377","url":"https:\/\/afsbio.com\/products\/nephrin-h892-polyclonal-antibody-bs3462","provider":"AFSBio Inc.","version":"1.0","type":"link"}