{"product_id":"nf2-polyclonal-antibody-bs80404","title":"NF2 Polyclonal Antibody-BS80404","description":"\u003cp\u003eNF2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS80404-50, BS80404-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35240\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eNF2 Polyclonal Antibody-BS80404\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 73KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using NF2 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 180s., Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF2 antibody. Western blot was performed from the immunoprecipitate using NF2 antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: NF2; ACN; BANF; SCH; merlin\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human NF2(NP_000259.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960143188147,"sku":"BS80404-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960143220915,"sku":"BS80404-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b5f5a6dd-6891-4ccc-b590-53879ccdaa29.jpg?v=1705231985","url":"https:\/\/afsbio.com\/products\/nf2-polyclonal-antibody-bs80404","provider":"AFSBio Inc.","version":"1.0","type":"link"}