{"product_id":"nfib-polyclonal-antibody-bs76230","title":"NFIB Polyclonal Antibody-BS76230","description":"\u003cp\u003eNFIB Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS76230-50, BS76230-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O00712\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eNFIB Polyclonal Antibody-BS76230\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:200 - 1:2000  |  IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 47kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of HeLa cells, using NFIB antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 120s., Immunohistochemistry of paraffin-embedded rat lung using NFIB Rabbit pAb at dilution of 1:250.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: NFIB; CTF; HMGIC\/NFIB; NF-I\/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human NFIB(NP_005587.2).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960042721459,"sku":"BS76230-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960042754227,"sku":"BS76230-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_7a3dab3f-78d3-45fd-a224-6e848cb2b94d.jpg?v=1705230884","url":"https:\/\/afsbio.com\/products\/nfib-polyclonal-antibody-bs76230","provider":"AFSBio Inc.","version":"1.0","type":"link"}