{"product_id":"nfkb-p100-polyclonal-antibody-bs90938","title":"NFkB p100 Polyclonal Antibody-BS90938","description":"\u003cp\u003eNFkB p100 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90938-50, BS90938-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q00653\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eNFkB p100 Polyclonal Antibody-BS90938\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:5000~1:10000\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: NFkB p100 polyclonal antibody detects endogenous levels of NFkB p100 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of NFkB p100 pAb at 1:5000 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: U-87MG whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: C6 whole cell lysate (40ug), Immunofluorescence analysis of A549 cells using NFkB p100 antibody at dilution of 1:50.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Lyt10; NFKB2; LYT10; CVID10; DNA binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; NF kB2; NFKB p52\/p100 subunit; Nuclear factor Kappa B subunit 2; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49\/p100); Nuclear factor of kappa light polypeptide gene enhancer in B cells 2; Oncogene Lyt 10; p100; Transcription factor NFKB2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein.\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962728091827,"sku":"BS90938-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962728124595,"sku":"BS90938-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f333dec9-70cd-4e22-9f17-298b0168da70.jpg?v=1705318603","url":"https:\/\/afsbio.com\/products\/nfkb-p100-polyclonal-antibody-bs90938","provider":"AFSBio Inc.","version":"1.0","type":"link"}