{"product_id":"nibrin-phospho-s343-polyclonal-antibody-bs4153","title":"Nibrin (phospho-S343) Polyclonal Antibody-BS4153","description":"\u003cp\u003eNibrin (phospho-S343) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4153-50, BS4153-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O60934\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eNibrin (phospho-S343) Polyclonal Antibody-BS4153\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11\/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-p95\/NBS1 (S343) polyclonal antibody detects endogenous levels of p95\/NBS1 protein when phosphorylated at Ser343.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 95 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-Nibrin (S343) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: HEK293T treated with UV for 5 minutes then repair for 1 hours whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: HEK293T treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-p95\/NBS1 (S343) pAb in paraffin-embedded human tonsil tissue.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; NBS1\/P95; NBS; NBS1; P95\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Nibrin around the phosphorylation site of Serine 343.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957296107699,"sku":"BS4153-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957296140467,"sku":"BS4153-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a25da843-70a0-493a-bb27-d766df7efad3.jpg?v=1705144500","url":"https:\/\/afsbio.com\/products\/nibrin-phospho-s343-polyclonal-antibody-bs4153","provider":"AFSBio Inc.","version":"1.0","type":"link"}