{"product_id":"nle1-y333-polyclonal-antibody-bs3353","title":"NLE1 (Y333) Polyclonal Antibody-BS3353","description":"\u003cp\u003eNLE1 (Y333) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3353-50, BS3353-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Notchless protein homolog 1; NLE1; HUSSY-07\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9NVX2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: NLE1 (Y333) polyclonal antibody detects endogenous levels of NLE1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 48 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of NLE1 (Y333) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: HEK293T whole cell lysate(20ug)\u003cbr\u003e\nLane 2: K562 whole cell lysate(20ug)\u003cbr\u003e\nLane 3: HCT116 whole cell lysate(20ug)\u003cbr\u003e\nLane 4: PC12 whole cell lysate(40ug)\u003cbr\u003e\nLane 5: CT26 whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 302-350 of Human NLE1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911804194995,"sku":"BS3353-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911804227763,"sku":"BS3353-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_86472f0a-e399-4d64-b586-ff1ee0fb9ce7.jpg?v=1703179568","url":"https:\/\/afsbio.com\/products\/nle1-y333-polyclonal-antibody-bs3353","provider":"AFSBio Inc.","version":"1.0","type":"link"}