{"product_id":"nlrp3-polyclonal-antibody-bs90948","title":"NLRP3 Polyclonal Antibody-BS90948","description":"\u003cp\u003eNLRP3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90948-50, BS90948-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96P20\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA IHC IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500-1:2000\u003cbr\u003e\nELISA: 1:5000-1:10000\u003cbr\u003e\nIHC: 1:100-1:500\u003cbr\u003e\nIF: 1:100-1:500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cryopyrin interacts selectively with apoptosis associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA\/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: NLRP3 polyclonal antibody detects endogenous levels of NLRP3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 114 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Sample:\u003cbr\u003e\n Lung (Rat) Lysate at 40 ug\u003cbr\u003e\n Colon carcinoma (Human) Lysate at 40 ug\u003cbr\u003e\n Primary: Anti-NALP3 at 1\/300 dilution, Tissue\/cell: Rat tracheal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;\u003cbr\u003e\n Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cryopyrin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: LRR and PYD domains-containing protein 3; AGTAVPRL; AII\/AVP antibody Angiotensin\/vasopressin receptor AII\/AVP like; Angiotensin\/vasopressin receptor AII\/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3; PYPAF 1; PYPAF1 antibody PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: KLH conjugated synthetic peptide derived from human Cryopyrin: 551-630\/1036\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42975429787827,"sku":"BS90948-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42975429820595,"sku":"BS90948-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c3d174aa-5c1e-46b7-8d95-af52708cde38.jpg?v=1705665934","url":"https:\/\/afsbio.com\/products\/nlrp3-polyclonal-antibody-bs90948","provider":"AFSBio Inc.","version":"1.0","type":"link"}