{"product_id":"nmi-monoclonal-antibody-mb67088","title":"Nmi Monoclonal Antibody-MB67088","description":"\u003cp\u003eNmi Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB67088-50, MB67088-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13287\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/10 - 1\/50), FC (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Acts as a signaling pathway regulator involved in innate immune system response.\u003cbr\u003e\nIn response to interleukin 2\/IL2 and interferon IFN-gamma\/IFNG, interacts with signal transducer and activator of transcription\/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis.\u003cbr\u003e\nEnhances the recruitment of CBP\/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription.\u003cbr\u003e\nIn response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35.\u003cbr\u003e\nIn complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21.\u003cbr\u003e\nIn complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65\/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries .\u003cbr\u003e\nNegatively regulates virus-triggered type I interferon\/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses (By similarity).\u003cbr\u003e\nBeside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion.\u003cbr\u003e\nMacrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4\/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of Nmi protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 40 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Nmi expression in A431 (A) whole cell lysates., Immunofluorescent analysis of Nmi staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: N-myc-interactor; Nmi; N-myc and STAT interactor\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human Nmi. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989548437683,"sku":"MB67088-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989548470451,"sku":"MB67088-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_daf2fe9b-f832-4675-97b8-f601d63af64d.jpg?v=1706095164","url":"https:\/\/afsbio.com\/products\/nmi-monoclonal-antibody-mb67088","provider":"AFSBio Inc.","version":"1.0","type":"link"}