{"product_id":"non-esterified-free-fatty-acids-nefa-colorimetric-assay-kit-e-bc-k014","title":"Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit - E-BC-K014","description":"\u003cp\u003eNon-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSize: 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: E-BC-K014-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: NEFA\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Microplate reader, Biochemistry analyzer\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, plasma, tissue homogenate, cells, cell supernatant\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 30 min\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colorimetric method\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.01-3.0 mmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS) . Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD) . Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD) . The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.\u003c\/p\u003e\n\n\u003cp\u003eStorage: This product can be stored at 2~8°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"96T","offer_id":43047604748467,"sku":"E-BC-K014-96","price":722.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Screenshot_2024-02-12_153513_39fec5a8-87b1-4a3b-ae7f-2f48465de893.png?v=1707825758","url":"https:\/\/afsbio.com\/products\/non-esterified-free-fatty-acids-nefa-colorimetric-assay-kit-e-bc-k014","provider":"AFSBio Inc.","version":"1.0","type":"link"}