{"product_id":"nrf2-acetyl-lys599-rabbit-polyclonal-antibody-es1138","title":"Nrf2 (Acetyl Lys599) Rabbit Polyclonal Antibody - ES1138","description":"\u003cp\u003eNrf2 (Acetyl Lys599) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1138-50, ES1138-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],\u003c\/p\u003e \n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized acetyl-peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599.\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Nrf2,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when acetylation at K599.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. IHC-p: 1:100-300 ELISA: 1\/10000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 75-100kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Protein processing in endoplasmic reticulum, Parkinson disease, Pathways in cancer, Chemical carcinogenesis - reactive oxygen species, Hepatocellular carcinoma, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis\u003c\/p\u003e\n\n\u003cp\u003eFunction: Domain: Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. PTM: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. Similarity: Belongs to the bZIP family. CNC subfamily. Similarity: Contains 1 bZIP domain. Subcellular Location: Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electr\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm, cytosol Nucleus Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: NFE2L2\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Nuclear factor erythroid 2-related factor 2\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 4780\u003c\/p\u003e\n\n\u003cp\u003eHuman SwissProt Number: Q16236\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 18024\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q60795\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 83619\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: O54968\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825501855923,"sku":"ES1138-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825501888691,"sku":"ES1138-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/nrf2-acetyl-lys599-rabbit-polyclonal-antibody-es1138","provider":"AFSBio Inc.","version":"1.0","type":"link"}