{"product_id":"nsd3-polyclonal-antibody-bs90970","title":"NSD3 Polyclonal Antibody-BS90970","description":"\u003cp\u003eNSD3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90970-50, BS90970-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9BZ95(Human) Q6P2L6(Mouse) EntrezGene:290831(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: NSD3 polyclonal antibody detects endogenous levels of NSD3 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 162 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: MCF-7 \u003cbr\u003e\n Lane 2: 293T, Flow cytometric analysis of Hela cells with NSD3 antibody at 1\/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: WHSC1-like protein 1, WHSC1L1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, Histone-lysine N-methyltransferase NSD3, MGC126766, MGC142029, NSD 3, NSD3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, Nuclear SET domain-containing protein 3, pp14328, Protein whistle, Whistle, WHSC1 like protein 1, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, WHSC1L1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1, Wolf-Hirschhorn syndrome candidate 1-like protein 1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984602796211,"sku":"BS90970-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984602828979,"sku":"BS90970-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_fae0e460-95dd-44a0-9505-e153711009cd.jpg?v=1705919160","url":"https:\/\/afsbio.com\/products\/nsd3-polyclonal-antibody-bs90970","provider":"AFSBio Inc.","version":"1.0","type":"link"}