{"product_id":"nsun3-polyclonal-antibody-bs75075","title":"NSUN3 Polyclonal Antibody-BS75075","description":"\u003cp\u003eNSUN3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS75075-50, BS75075-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H649\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C at position 34 of mt-tRNA (Met. mt-tRNA (Met methylation at cytosine (34 takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f (5c at this position. mt-tRNA (Met containing the f (5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 38kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using NSUN3 antibody at 1:3000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 30s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: NSUN3; MST077; MSTP077\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human NSUN3 (NP_071355.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42920243921075,"sku":"BS75075-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42920243953843,"sku":"BS75075-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_aae834ed-b3c2-48e1-a075-1467bd84a4a8.jpg?v=1703675247","url":"https:\/\/afsbio.com\/products\/nsun3-polyclonal-antibody-bs75075","provider":"AFSBio Inc.","version":"1.0","type":"link"}