{"product_id":"occludin-a23-polyclonal-antibody-ap0765","title":"Occludin (A23) Polyclonal Antibody-AP0765","description":"\u003cp\u003eOccludin (A23) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0765-50, AP0765-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16625\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Occludin (A23) polyclonal antibody detects endogenous levels of Occludin protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Occludin (A23) polyclonal antibody at 1:1000 dilution\u003cbr\u003e\nLane1:Hela whole cell lysate(40ug)\u003cbr\u003e\nLane2:PC12 whole cell lysate(40ug)\u003cbr\u003e\nLane3:The Kidney tissue lysate of Mouse(30ug)\u003cbr\u003e\nLane4:The Kidney tissue lysate of Rat(30ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 59 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: OCLN\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to residues in Human Occludin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910473355443,"sku":"AP0765-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910473388211,"sku":"AP0765-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_23abe805-aaa9-4d95-93c1-1aa66f6135b9.jpg?v=1703093115","url":"https:\/\/afsbio.com\/products\/occludin-a23-polyclonal-antibody-ap0765","provider":"AFSBio Inc.","version":"1.0","type":"link"}