{"product_id":"occludin-polyclonal-antibody-bs72035","title":"Occludin Polyclonal Antibody-BS72035","description":"Occludin Polyclonal Antibody\n\nProduct: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS72035-50, BS72035-100\n\nSwiss-Prot: Q16625\n\nHost: Rabbit\n\nOccludin Polyclonal Antibody-BS72035\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC, IF ICC ELISA\n\nAll Applications: WB\t 1:500 - 1:2000\nIHC\t 1:100 - 1:500\nIF\t 1:100 - 1:500\nICC 1:100 - 1:500\nELISA 1:5000 - 1:10000\n\nBackground: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nSpecificity: Occludin polyclonal antibody detects endogenous levels of Occludin protein.\n\nBioworld Molecular Weight: ~ 59 kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Sample:\n Testis (Mouse) Lysate at 40 ug\n Kidney (Mouse) Lysate at 40 ug\n Primary: Anti-Occludin at 1\/300 dilution, Paraformaldehyde-fixed, paraffin embedded (Normal skin)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.\n\nAlternative Name: OCLN; Occludin; BLCPMG\n\nImmunogen: KLH conjugated synthetic peptide derived from human Occludin:151-250\/522 \u003cextracellular\u003e\n\nModification: Unmodified\n\nConjugate: Unconjugated\u003c\/extracellular\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962728878259,"sku":"BS72035-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962728911027,"sku":"BS72035-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d856ed3b-42f9-4ba7-b5ed-5ac26352a946.jpg?v=1705318607","url":"https:\/\/afsbio.com\/products\/occludin-polyclonal-antibody-bs72035","provider":"AFSBio Inc.","version":"1.0","type":"link"}