{"product_id":"occludin-polyclonal-antibody-bs90980","title":"Occludin Polyclonal Antibody-BS90980","description":"\u003cp\u003eOccludin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS90980-50, BS90980-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16625(Human) Q61146(Mouse) Q6P6T5(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Flow cytometric analysis of 293 cells with Occludin antibody at 1\/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as theSecondary antibody.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 59 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Occludin polyclonal antibody detects endogenous levels of Occludin protein.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: OCLN, BLCPMG, FLJ08163, FLJ18079, FLJ77961, FLJ94056, MGC34277, Occludin, Ocln, OCLN, Tight junction protein occludin,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939204796595,"sku":"BS90980-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939204829363,"sku":"BS90980-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c027d16d-c011-424d-a393-a4d0e043958b.jpg?v=1704545057","url":"https:\/\/afsbio.com\/products\/occludin-polyclonal-antibody-bs90980","provider":"AFSBio Inc.","version":"1.0","type":"link"}