The Omalizumab PK ELISA kit is designed to measure Omalizumab in serum or plasma with high specificity and sensitivity through sandwich technique.
The Omalizumab PK ELISA kit is designed to measure Omalizumab in serum or plasma with high specificity and sensitivity. This sandwich ELISA design employs a Omalizumab specific monoclonal antibody for capture and a specific monoclonal antibody for detection.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti- Omalizumab is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Omalizumab present in biological matrices is bound by the immobilized anti- Omalizumab antibody. After washing away any unbound substances, enzyme linked anti- Omalizumab antibody is added to the wells. This antibody is developed and purified specifically against Xolair® (domain residing in Fc portion of the Xolair® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Omalizumab present in test samples. The color development is stopped and the intensity of the color is measured.
CATALOGUE NUMBER: EL-1611-152
Peroxidase / OD450
STORAGE AND STABILITY
Stable at -20°C for 1 year
SAFETY WARNINGS AND PRECAUTIONS
Research Use Only. Follow instructions.
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
EACH KIT INCLUDES:
Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (250 ug/mL) - 12ul
10X wash buffer - 25ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3